It’s time consuming to set up and use FACS for my sorting. How does set up time for the Pala Cell Sorter compare to traditional FACS?
设置和使用FACS进行分类非常耗时。Pala 细胞分选仪的设置时间与传统 FACS 相比如何？
Preparing and maintaining the instrument before and after the actual experiment—also known as overhead time—is the Achilles heel of many standard analytical instruments. The Pala Single Cell Dispenser drastically reduces overhead time, letting you go from initializing the instrument to achieving single cell isolation in as little as 7 minutes. This is in contrast to conventional FACS systems, which take over an hour to operate and provide results.
在实际实验之前和之后准备和维护仪器（也称为开销时间）是许多标准分析仪器的致命弱点。Pala 单细胞分液器大大缩短了开销时间，让您在短短 7 分钟内完成从初始化仪器到实现单细胞分离的整个过程。这与 传统的FACS系统形成鲜明对比，后者需要一个多小时才能运行并提供结果。
FACS and limited dilution often yield few viable cells. Can the Pala Single and Bulk Cell Sorter help me achieve healthy cells after sorting?
You can rest assured that the cells you get from the Pala system will be intact, healthy, and viable. The Pala system is gentle and uses very low pressure (<2 psi) for cell sorting, in contrast to high pressures (30-70 psi) typically used by conventional FACS sorters. The figure on the left shows a comparison of cellular stress response to pressures from FACS and Pala. Cells sorted with a FACS instrument showed induction of cellular stress response genes such as Ubiquitin C (Ubc), likely owing to high-pressure sorting conditions, while cells sorted on the Pala system showed much lower expression of these genes.
您可以放心，您从 Pala 系统获得的细胞将是完整、健康和可行的。 Pala 系统非常温和，使用非常低的压力 （<2 psi） 进行细胞分选，而传统 FACS 分选机通常使用的高压 （30-70 psi）。左图显示了细胞应激反应对 FACS 和 Pala 压力的比较。用FACS仪器分选的细胞显示出细胞应激反应基因的诱导，如泛素C（Ubc），这可能是由于高压分选条件，而在Pala系统上分选的细胞显示出这些基因的表达要低得多。
Having to sort my prepared cells in a different lab complicates my experimental procedure and adds uncertainty to the outcome.
必须在不同的实验室中对我制备的细胞进行分类，使我的实验过程复杂化，并增加了结果的不确定性。
Pala is a compact, benchtop system which can easily fit into your tissue culture hood for sterile sorting. You can prepare your cells and easily isolate them in the same workstation!
Pala 是一种紧凑的台式系统，可以轻松安装到您的组织培养罩中进行无菌分选。您可以在同一工作站中制备细胞并轻松分离它们！
The cell sorting step that happens on a FACS sorter exposes my cells to contamination risks. How does the Pala cell sorter mitigate contamination risk in my lab?
Pala and other single cell sorters from Namocell utilize disposable . Specifically designed for sterile isolation, each cartridge is a closed system, preventing cross contamination and aerosol formation.
Pala 和 Namocell 的其他单细胞分选仪 使用一次性微流控细胞盒。专为无菌隔离而设计，每个滤芯都是一个封闭系统，可防止交叉污染和气溶胶形成。
I am concerned about learning how to use a complex instrument.
我担心学习如何使用复杂的仪器。
The Pala system is automated and easy-to-use. It streamlines many of the steps required for traditional single cell sorters, therefore it doesn’trequire any extensive training or specialized expertise. Anyone can run it!
Pala 系统是自动化且易于使用的。它简化了传统单细胞分选仪所需的许多步骤，因此 不需要任何广泛的培训或专业知识。任何人都可以运行它！
